8. Limits of detection (LOD) and quantification (LOQ)
The limits of detection and quantification were determined by serial dilutions of ascorbic acid solutions in order to obtain signal/noise ratios of ≈3:1 for LOD and ≈10:1 for LOQ.
Approximately 25 mg of standard ascorbic acid were weighed precisely and dissolved in 50 mL of the mobile phase. Appropriate amounts of standard ascorbic acid solution were diluted to required concentrations of 0.03, 0.05, 0.1 and 1.0_gmL−1.Working standard solutions were prepared in replicates of three.