The objective of this work was to evaluate in vitro and in vivo biocontrol of bacterial spot (Xanthomonas
vesicatoria) and early blight (Alternaria solani) by the epiphytic bacteria Paenibacillus macerans and Bacillus
pumilus. Tomato plants were previously sprayed with epiphytic bacteria, benzalkonium chloride and PBS buffer
and, after four days, they were inoculated with A. solani and X. vesicatoria. To determine the phytopathogenic
bacteria population, leaflet samples were collected from each treatment every 24 hours, for seven days, and
plated on semi-selective medium. The effect of epiphytic bacteria over phytopathogens was performed by the
antibiosis test and antagonistic activity measured by inhibition zone diameter. The epiphytic and benzalkonium
chloride drastically reduced the severity of early blight and bacterial spot in comparison to the control (PBS).
In detached leaflets, the epiphytic bacteria reduced in 70% the number of phytopathogenic bacteria cells in
the phylloplane. The antibiosis test showed that the epiphytic bacteria efficiently inhibit the phytopathogens
growth. In all the bioassays, the epiphytic bacteria protect tomato plants against the phytopathogens
The objective of this work was to evaluate in vitro and in vivo biocontrol of bacterial spot (Xanthomonas vesicatoria) and early blight (Alternaria solani) by the epiphytic bacteria Paenibacillus macerans and Bacillus pumilus. Tomato plants were previously sprayed with epiphytic bacteria, benzalkonium chloride and PBS buffer and, after four days, they were inoculated with A. solani and X. vesicatoria. To determine the phytopathogenic bacteria population, leaflet samples were collected from each treatment every 24 hours, for seven days, and plated on semi-selective medium. The effect of epiphytic bacteria over phytopathogens was performed by the antibiosis test and antagonistic activity measured by inhibition zone diameter. The epiphytic and benzalkonium chloride drastically reduced the severity of early blight and bacterial spot in comparison to the control (PBS). In detached leaflets, the epiphytic bacteria reduced in 70% the number of phytopathogenic bacteria cells in the phylloplane. The antibiosis test showed that the epiphytic bacteria efficiently inhibit the phytopathogens growth. In all the bioassays, the epiphytic bacteria protect tomato plants against the phytopathogens
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