Gelation of the collagen-suspended cells (2 ml gel/well; Falcon 6-well culture plates,
Falcon Plastics, Los Angeles, CA) required 5 mm at 4#{176}C;cells (approximate final concentration of 250,000 cells/mi) were then overlaid with an additional
2 ml of MEM ± 8pM colchicine (to give a final concentration of 4pM) and incubated as
indicated below.