abstract
This paper reports the findings of the ongoing studies on cryopreservation of the snakehead,Channa
striataembryos. The specific objective of this study was to collect data on the sensitivity ofC. striata
embryo hatching rate to low temperatures at two different developmental stages in the presence of four
different cryoprotectants. Embryos at morula and heartbeat stages were selected and incubated in 1 M
dimethyl sulfoxide (Me2SO), 1 M ethylene glycol (EG), 1 M methanol (MeOH) and 0.1 M sucrose solutions
at different temperatures for a period of time. Embryos were kept at 24C (control), 15C, 4C and2C
for 5 min, 1 h and 3 h. Following these treatments, the embryos were then transferred into a 24C water
bath until hatch to evaluate the hatching rate. The results showed that there was a significant decrease of
hatching rate in both developmental stages following exposure to 4C and2C at 1 h and 3 h exposure
in each treatment. Heartbeat stage was more tolerant against chilling at2C for 3 h exposure in Me2SO
followed by MeOH, sucrose and EG. Further studies will be conducted to find the best method to preserve
embryos for long term storage.
abstract
This paper reports the findings of the ongoing studies on cryopreservation of the snakehead,Channa
striataembryos. The specific objective of this study was to collect data on the sensitivity ofC. striata
embryo hatching rate to low temperatures at two different developmental stages in the presence of four
different cryoprotectants. Embryos at morula and heartbeat stages were selected and incubated in 1 M
dimethyl sulfoxide (Me2SO), 1 M ethylene glycol (EG), 1 M methanol (MeOH) and 0.1 M sucrose solutions
at different temperatures for a period of time. Embryos were kept at 24C (control), 15C, 4C and2C
for 5 min, 1 h and 3 h. Following these treatments, the embryos were then transferred into a 24C water
bath until hatch to evaluate the hatching rate. The results showed that there was a significant decrease of
hatching rate in both developmental stages following exposure to 4C and2C at 1 h and 3 h exposure
in each treatment. Heartbeat stage was more tolerant against chilling at2C for 3 h exposure in Me2SO
followed by MeOH, sucrose and EG. Further studies will be conducted to find the best method to preserve
embryos for long term storage.
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