2.5. Scanning electron microscopy Samples for scanning electron microscopy (SEM) were prepared according to the method described by Sidhu, Seibel, and Meyer (1990) with slight modifications. The dough sheets containing the hydrocolloids, used for frying were cut into small pieces of 0.5 0.5 mm, freeze-dried using a Heto freeze dryer (model DW3, 192 K.R. Parimala, M.L. Sudha / Food Hydrocolloids 27 (2012) 191e200 Denmark). The freeze-dried pieces of puri dough sheet and moisture and fat free puri samples were mounted on the specimen holder using double-sided scotch tape and were exposed to gold sputtering (2 min, 2 m bar). The samples were then subjected to scanning electron microscopic examination using a Leo scanning electron microscope, Model 435 VP (Leo Electron Microscopy Ltd., Cambridge, England) at an accelerating voltage of 15 kV with a vacuum of 9.75 10 5 Torr. Micrographs of appropriate magni- fications were selected for presentation of results.
2.5. Scanning electron microscopy Samples for scanning electron microscopy (SEM) were prepared according to the method described by Sidhu, Seibel, and Meyer (1990) with slight modifications. The dough sheets containing the hydrocolloids, used for frying were cut into small pieces of 0.5 0.5 mm, freeze-dried using a Heto freeze dryer (model DW3, 192 K.R. Parimala, M.L. Sudha / Food Hydrocolloids 27 (2012) 191e200 Denmark). The freeze-dried pieces of puri dough sheet and moisture and fat free puri samples were mounted on the specimen holder using double-sided scotch tape and were exposed to gold sputtering (2 min, 2 m bar). The samples were then subjected to scanning electron microscopic examination using a Leo scanning electron microscope, Model 435 VP (Leo Electron Microscopy Ltd., Cambridge, England) at an accelerating voltage of 15 kV with a vacuum of 9.75 10 5 Torr. Micrographs of appropriate magni- fications were selected for presentation of results.
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