Soil samples were taken with the help of sterilized spatula at a depth of 5–15 cm from agricultural fields in menofia governorate egypt cultivated with seedlings of Vicia faba treated with plant growth hormone (gibberellic acid), insecticide Cyperkill containing active ingredient Cypermethrin, fungicide Topas containing active ingredient Penconazole, mixture of insecticide with plant growth hormone and mixture of fungicide with plant growth hormone at field application rates. One gm of soil was mixed with 9 ml of sterilized water and mixed thoroughly. 1 ml from the solution was then mixed in 9 ml sterilized water to make 102 dilution of this solution and in the same pattern dilutions up to 107 were prepared to determine the microbial count