The increase in intracellular neutral lipid during the growth
cycle of isolated microalgae strains was measured via fluorescence
intensity ofNR stained cultures [12] simultaneously to cell
growth. 10 mL of NR was added to 3mL of algal suspensions
whichwas thenvortexed for 1min prior to incubationfor 10min
in darkness at room temperature. NR emits a yellow-gold fluorescence
when dissolved in neutral lipid which is the best
substrate to produce biodiesel