The same eggs were used for internal quality measurement. Shell thickness was measured by Peacock dial pipe gauge FHK (Ozaki, Meg. Co., Ltd, Japan, Japan) and is presented as the average thickness of three locations on the egg (air cell, equator and sharp end). For yolk color determination, egg albumen, eggshell, and shell membranes were removed from broken eggs and the egg yolk color was measured was flushed under a flow of nitrogen and sealed. The sealed ampoules were heated in a water bath at 95°C for 45 min and cooled.