At 0 and 4 h post-feeding, rumen fluids were collected
and prepared for DNA extraction for total bacteria, methanogens
and predominant cellulolytic bacteria (Fibrobacter
succinogenes, Ruminococcus flavefaciens, and Ruminococcus
albus). Total bacteria, methanogenic bacteria and predominant
cellulolytic bacteria population were determined by
using real-time PCR. All primer set sequences (forward and
reverse primers) are shown in Table 2.