Figure 4 shows TEM micrographs taken at different fer-
mentation times. The evolution of the cells and their
polymer inclusions can be observed. Micrographs of
samples after 4 h of fermentation show long, growing cells, while 12-h grown cells are already synthesizing poly-
mer. Micrographs taken from samples of 21 h of fermen-
tation show shorter and wider cells deformed due to the
pressure of the big internal inclusions. In this last case,
several difficulties were encountered in cutting off the
samples with the microtome due to the high amount,
and hence, resistance of the intracellular inclusions. Con-
sequently, the cellular walls of several cells were fractured
because of the jump of some internal inclusions. This can
be observed in the micrographs of the samples at 12 and
21 h of fermentation (black arrow in Fig. 4).