The inoculum was prepared by inoculating a twoday
old slant culture into 25 ml of the sterile MPYD
liquid medium taken in 100 ml flask and growing it on a
rotary shaker (100 rev min)1
) for 48 h. This inoculum
(3 · 106 cells ml)1
) was used in the fermentation of
mango must (juice) to mango wine.