Conversely, the maximum amount of total protein was
produced using the M2 medium followed by the M4 medium,
from which 39% less protein was obtained. Using
the M3 medium, 43% less protein was obtained than
with the M2 medium, and finally, the lowest amount of
total protein was obtained using the M1 medium, which
was 62% less than with the M2 medium. The variation
in the amount of total protein obtained could be due to
the concentration of crude protein and the amount of
amino acids in the media, particularly arginine and glutamic
acid, which are important for protein synthesis.