Each amplification with ISSR and SRAP primers was performed
in a 25 mL reaction volume containing 20 mM Tris–HCl (pH 8.4),
50 mM KCl, 2 mM MgCl2,800mM dNTP (dATP:dTTP:dCTP:dGTP in
a 1:1:1:1 ratio), 0.5mM of each primer, 1 U of PlatinumTaqpolymerase (Invitrogen, Life Technologies)