3.2. Determination of the figures of merit
The proposed method was validated by considering the linear
dynamic range, repeatability, limit of detection (LOD), limit of quantification
(LOQ), precision, interferences, and recovery.
The analytical curve, which was linear in the range 1.65 to
9.00 mmol L−1, was constructed by appropriate dilutions of thefurosemide stock solution. A blank was prepared containing 20 μL of
the chromogenic reagent and 20 μL of ethanol. The regression equation
obtained was