The B. subtilis cells were harvested from overnight bacterial cultures in Luria–Bertani broth (LB)by centrifugation at 8,000 rpm, and were streaked approximately 1 cm away from the growing edge of 2 day-old fungal colony. The cell-free supernatant was collected by centrifugation of a 3 day-old bacterial culture in LB, and sterilized by autoclaving at 121◦C for 15 min.