Saccharomyces cerevisiae (baker's yeast) due to its successful use in different biotechnological processes and industries such as in bakery and brewery is an efficient microorganism. However, limi- tations for the fermentation of lignocellulosic hydrolysate products, mainly pentose and other components have been found to be the main hurdles in maintaining higher yields of ethanol. Possible ge- netic modification by recombinant DNA technology and compre- hensive genome information has currently made this microorganism more attractive to increase ethanol production (Ostergaard et al., 2006). In this study, microorganisms without any prior modification were used in DHF for the conversion of the soluble products of cellulose and sugars of CER to ethanol. Although in most ethanol production processes different treatments and temperatures are needed for separate hydrolysis and fermentation, operation under distinct and lower temperatures of the CER in the presence of the free saccharomyces cerevisiae is desirable from process point of view and cost effectiveness.