There is limited literature about green color extracts and stability.
Senklang and Anprung (2009) studied extraction of chlorophyll
either enzymatically or non-enzymatically from the pandan leaf.
This previous research laid the groundwork for studying green
color extraction from high chlorophyll content spinach, and
improvement of chlorophyll stability. With this aim, the first objective
of this study was to determine optimum conditions; pH, zinc
chloride (ZnCl2) concentration, temperature and duration on the
formation of stable green colored Zn–chlorophyll derivatives from
spinach leaves. The second objective was to optimize the release of
Zn–chlorophyll derivatives from pulp with enzyme pretreatment
by using response surface methodology (RSM), which allows the
concurrent evaluation of different process variables such as temperature,
time, enzyme concentration, and variable interactions
on total chlorophyll content (TCC) in the extract. RSM was also
used to compare the efficiency of solvent and enzyme-assisted
extractions.