At Zadoks growth stages (ZGS) (45 booting), 65 (50% anthesis), and 92 (grain ripe), five randomly-selected plants from each plot were sampled (Zadoks et al., 1974). The plants were washed and separated into the upper and lower leaves of the main stem for dry weight measurements, ionic analysis, leaf osmotic potential, and organic solutes as explained in Experiment 1.