CFE-SEM images of the CBs, GA-CBs, and LA-GA-CBs are shown
in Fig. 4. The porous and rough structure of the CBs is easily recognized
in Fig. 4a. This structure reveals the high biosorption capability
of CBs. Before and after laccase immobilization, the surface
structure of GA-CBs clearly changes (Fig. 4b and c). On the surface
of GA-CBs, the rippled and rough structure was replaced by a
smoother and tighter structure. These images show that a stable
polymeric structure of a laccase layer was covered on the surface
of the GA-CBs and that the immobilization of laccase on GA-CBs
was effective.