Operation strategy
The SBRs were operated with a cycle of 6 h. The cycle consisted
of 5 phases: 1 h of influent feeding, 3 h of aeration, 1 h of settling,
10 min of drawing, and 50 min of idle period, which was consistent
with the actual operation of the WWTP. The volumetric exchange
ratio was 50% and dissolved oxygen (DO) was maintained at
2–3 mg/L. After one week stabilization, the two reactors were
operated with different protocols: (1) R1, control SBR, with no
extra bacteria addition; (2) R2, bioaugmented SBR, seeded with
3 g PCN bacteria made in 2.1. The operation was divided into two
phases, first phase (0–20 d): both reactors were fed with raw
wastewater; second phase (21–53 d): sodium succinate was used
as the extra carbon source and directly added to the reactor at
the beginning of every aeration period. The pH and DO values were
monitored at a regular time interval during the operation.
For long operation of reactor R2, 50 g of sodium succinate was
directly added to the reactor at the beginning of every aeration
period. The solid retention time (SRT) of reactor R2 was kept at
12–15 d and the mixed liquor suspended solids (MLSS) in the main
Operation strategyThe SBRs were operated with a cycle of 6 h. The cycle consistedof 5 phases: 1 h of influent feeding, 3 h of aeration, 1 h of settling,10 min of drawing, and 50 min of idle period, which was consistentwith the actual operation of the WWTP. The volumetric exchangeratio was 50% and dissolved oxygen (DO) was maintained at2–3 mg/L. After one week stabilization, the two reactors wereoperated with different protocols: (1) R1, control SBR, with noextra bacteria addition; (2) R2, bioaugmented SBR, seeded with3 g PCN bacteria made in 2.1. The operation was divided into twophases, first phase (0–20 d): both reactors were fed with rawwastewater; second phase (21–53 d): sodium succinate was usedas the extra carbon source and directly added to the reactor atthe beginning of every aeration period. The pH and DO values weremonitored at a regular time interval during the operation.For long operation of reactor R2, 50 g of sodium succinate wasdirectly added to the reactor at the beginning of every aerationperiod. The solid retention time (SRT) of reactor R2 was kept at12–15 d and the mixed liquor suspended solids (MLSS) in the main
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