Test for Recombination
To overcome the effects of recombination on phylogenetic
and evolutionary interpretations (Posada and Crandall 2002),
we employed GARD and Single Breakpoint algorithms
implemented in the HyPhy package and assessed recombination
on all the toxin forms examined in this study (Delport
et al. 2010; Kosakovsky Pond et al. 2006). When potential
breakpoints were detected using the small sample Akaike
information criterion (AICc), the sequences were compartmentalized
before conducting the selection analyses