The cultures were grown in Tryptic Soy Agar (Oxoid, Basingstoke, UK) for 18–24 h at 35 °C, and the colonies were suspended in sterile saline solution (0.85%) to reach a turbidity correspondent to 0.5 of the McFarland scale (108 UFC mL−1 )
The cultures were grown in Tryptic Soy Agar(Oxoid, Basingstoke, UK) for 18–24 h at 35 °C, and the colonieswere suspended in sterile saline solution (0.85%) to reach a turbiditycorrespondent to 0.5 of the McFarland scale (108 UFC mL−1)