All fecal samples were examined using FEC and microscopic examination. The samples were
also subjected to APC as described previously. In brief, 3–4 g of stool was placed on nutrient agar dish. After incubation at 28–30°C for 2–3 days, the dishes were evaluated by light
microscopy with low magnification for detection of any larva and adults or their tracks.7,8 For collection of larvae, the surface of the positive agar plate was washed out by lukewarm
phosphate buffer saline (PBS) solution.8 Differentiation ofS. stercoralis from other probable nematode larvae such asTrichostrongylus spp., hookworm, and also Rhabditis spp.
was carried out based on morphological characteristics
All fecal samples were examined using FEC and microscopic examination. The samples werealso subjected to APC as described previously. In brief, 3–4 g of stool was placed on nutrient agar dish. After incubation at 28–30°C for 2–3 days, the dishes were evaluated by lightmicroscopy with low magnification for detection of any larva and adults or their tracks.7,8 For collection of larvae, the surface of the positive agar plate was washed out by lukewarmphosphate buffer saline (PBS) solution.8 Differentiation ofS. stercoralis from other probable nematode larvae such asTrichostrongylus spp., hookworm, and also Rhabditis spp.was carried out based on morphological characteristics
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