treatment being killed on a given day. Lambs were
rendered unconscious by a nonpenetrating stunning
method. Immediately after stunning, four 1.27-cmdiameter
cores were removed from the longissimus
muscle (LM) perpendicular to the length of the LM
on the left side of the carcass. Two cores were removed
cranial to the 10th thoracic vertebra (designated as
foresaddle samples), and two cores were removed
caudal to the second lumbar vertebra (designated as
hindsaddle samples). Subsequently, LM samples were
removed at .75, 3, 6, 12, and 24 h after stunning. One
core was removed for pH determinations, and the
second core was frozen immediately in liquid NZ for
determinations of muscle glycogen and lactate concentrations
at a later date. The pH samples were
homogenized with sodium iodoacetate and analyzed
within 1 h. Carcasses were chilled conventionally at
2°C until fabricated into primal and retail cuts
treatment being killed on a given day. Lambs wererendered unconscious by a nonpenetrating stunningmethod. Immediately after stunning, four 1.27-cmdiametercores were removed from the longissimusmuscle (LM) perpendicular to the length of the LMon the left side of the carcass. Two cores were removedcranial to the 10th thoracic vertebra (designated asforesaddle samples), and two cores were removedcaudal to the second lumbar vertebra (designated ashindsaddle samples). Subsequently, LM samples wereremoved at .75, 3, 6, 12, and 24 h after stunning. Onecore was removed for pH determinations, and thesecond core was frozen immediately in liquid NZ fordeterminations of muscle glycogen and lactate concentrationsat a later date. The pH samples werehomogenized with sodium iodoacetate and analyzedwithin 1 h. Carcasses were chilled conventionally at2°C until fabricated into primal and retail cuts
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