3. Results
3.1. Transformation and regeneration
When leaf explants were inoculated with Agrobacterium immediately after excision,
kanamycin-resistant calli were produced on the selection medium after 5–7 weeks.
Adventitious shoots differentiated from the calli after 8–12 weeks of culture. About 90% of
leaf explants produced at least one callus. Non-inoculated explants did not survive on the
selection medium.