3. Results
3.1. Detection of YHV proteins in the hemolymph
and in primary cell cultures
Western blot analysis of YHV-containing
hemolymph samples obtained from time-course
experiments using experimentally infected shrimps
showed that the putative G protein (135 kDa) as
well as L protein (170 kDa) could be distinguished
specifically. Fig. 1 shows that the viral protein
first became detectable in the hemolymph at 64 h
post-infection (p.i.).
Western blot analysis of YHV-infected primary
lymphoid cell cultures had similar results. The
135-kDa protein of the virus was detectable as
early as 4 days