uncovered inoculated PDA plates were placed on a perforated plastic sheet inside hermetically closed plastic chambers (1.7-L capacity) with transparent lids, leaving sufficient headspace (each airtight chamber utilized was tested for leaks prior to experimenting with the essential oil). A glass plate containing the essential oil (500, 1000, 2000, 4000, 8000, or 16,000 mg of EO l1 of air) was located under the
perforated plastic sheet