2.6.2. Plant growth conditions and inoculation
Tomato seeds of genotypes KK 2 and L390 were sown in the greenhouse (20 °C, 14 h photoperiod per day, 30 K Lux and 70% RH). The roots of four-week-old tomato seedlings of each genotype was immersed in each bacterial suspensions for 60 min and transplanted to individual pots with approximately 300 g of soil (Fruhstorfer Erde, type P: 150 mg L−1 N, 150 mg L−1 P2O5, and 250 mg L−1 K2O). Potted seedlings were transferred to a climate chamber (30/28 °C day/night temperature, 14 h photoperiod, 30 K Lux, and 80% RH). Subsequently, 20 mL of each bacterial suspension was poured onto the substrate of each pot. Seedlings immersed in tap water were used as a control. After two days, the roots of each plant were artificially wounded and inoculated with R. solanacearum by pouring 25 mL of bacterial suspension per pot at the base of the plant.
2.6.2. Plant growth conditions and inoculation
Tomato seeds of genotypes KK 2 and L390 were sown in the greenhouse (20 °C, 14 h photoperiod per day, 30 K Lux and 70% RH). The roots of four-week-old tomato seedlings of each genotype was immersed in each bacterial suspensions for 60 min and transplanted to individual pots with approximately 300 g of soil (Fruhstorfer Erde, type P: 150 mg L−1 N, 150 mg L−1 P2O5, and 250 mg L−1 K2O). Potted seedlings were transferred to a climate chamber (30/28 °C day/night temperature, 14 h photoperiod, 30 K Lux, and 80% RH). Subsequently, 20 mL of each bacterial suspension was poured onto the substrate of each pot. Seedlings immersed in tap water were used as a control. After two days, the roots of each plant were artificially wounded and inoculated with R. solanacearum by pouring 25 mL of bacterial suspension per pot at the base of the plant.
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