Repetto and others introduced an improved IH DNA extrac-tion method based on efficient lysis of larvae and removal of inhibitors in the fecal samples.In this method, 1 g of stool is
used for extraction, whereas routine commercial DNA extrac-tion kits use only 200–300 mg stool. The DNA extraction pro-cess is a critical step affecting the efficacy of molecular
methods. In this study, the modified IH method was effi-ciently applied forS. stercoralisDNA extraction from stool samples. In fact, utilizing 1 g stool combined with GTES buffer plus mechanical disruption enables successful DNA extraction, even in cases of low infection, in which only few larvae were present in the stool samples