Briefly, the complete frac-tion (2 ml) of sporulated oocysts (obtained in Section2.3), was son-icated for 15 (53) minutes in a water-jacketed processing vessel
with cold water circulation and centrifuged for 10 min at 10,000g.
The supernatant (soluble antigen) was used as antigen to coat the
wells of microtitration plates.