An attempt was made in this study to use diluted wine as the aqueous phase and grapeseed oil as the oilphase for the preparation of oil-in-water cosmetic emulsions. Two monovarietal wines of Hellenic originwere used in this study; a red one from Sangiovese grapes and a white one from Muscat of Samos grapes.The oil-to-water ratio in the emulsions was 20:80 (v/v) and the wine concentrations in the aqueousphase were in the range of 5–100% (v/v). Glycerol monostearate was used as emulsifying agent. The onlyextra additive was mastic resin from Pistacialentiscus var. Chia, which is reported to have healing andantibacterial properties. The study of the emulsion stability involved droplet size determinations andviscosity measurements for a period of sixty days. It was found that the optimum percentage of wine(red and white) in the aqueous phase of such emulsions is 20% (v/v) and of the mastic resin 2% (w/v).