Figure 6 Detection of a-tubulin, ELSPBP1, PSMA6, AK1, PEBP1 and
BSP1 in the immotile (I) and motile (M) sperm subpopulations of
frozen–thawed semen from three bulls. Immotile and motile sperm
subpopulations were separated following centrifugation of frozen–
thawed semen on discontinuous Percoll gradient. Qualitative sperm
motility was assessed visually. Each line contains protein extracts from
3!106 cells.