The aim of the present study was to develop TiO2-coated oriented-polypropylene (OPP) film, clarify its antimicrobial activity and causative factors and assess its potential use in food packaging. The TiO2-coated OPP packaging films were developed with two different particle sizes of TiO2 powder. The antimicrobial capacity of TiO2-coated packaging film against E. coli was examined both in vitro and in actual tests under two kinds of artificial light.