Detection of antagonistic activity
Conventional streak method described by Fakoya et al,
(2009) was used for this assay. With the use of a sterile
cork borer of 7 mm diameter, an actively growing edge of
a 4-day old mycelial culture of P. ostreatus was aseptically
cut and placed at the center of a PDA plate. Forty
millimeter streak of test organisms (P. tolaasii and K.
macalapinaeae ) was made from a 24 h and 72 h old
culture of P. tolaasii and K. macalapinaeae respectively,
23 mm away from the center of the Petri-dish. The plates
were incubated for 168 h at 25 °C for the fungus and 37
°C for the bacterium respectively. The percentage
inhibition and inter-colony distance were measured on
daily basis.