One gram of germinating seed was ground in 10 mL of 0.1 M acetate buffer, pH 4.75, homogenized for 30 min, and centrifuged at 10,000 rpm for 10 min at 4 C. The supernatant was used for the enzyme assay. Enzyme extract (0.25 mL) was taken and diluted to 1 mL. One milliliter of a 0.01% (w/v) starch solution