Figure 4 Wnt pathway members are expressed in the episphere and hyposphere during early development.
The prototroch is marked with a dashed line. The tip of the apical plate is marked with an asterisk. Arrows indicate dorsal (D), ventral (V), anterior (A) and posterior (P).(A) Frizzled 5/8 across the apical plate, 16 hours post-fertilization (hpf) apical view and a(A’) 20 hpf ventral view (inset shows a surface ventral view).(B) Wnt4 along the blastopore, 16 hpf lateral view.(B’) Confocal image of ventral view of Wnt4 expression in which antibody staining to acetylated tubulin is visible with a green secondary antibody, reflection signal of in situ hybridization is red and nuclei are visible, stained with DAPI, in blue.(C) Apical view of sfrp1/5 expression at 24 hpf and at(C’) 48 hpf.(A”,B”and C”) Schematic representations of the expression patterns in(A) and(B) and(C) and(A’) and(B’) and(C’).
Figure 4 Wnt pathway members are expressed in the episphere and hyposphere during early development.The prototroch is marked with a dashed line. The tip of the apical plate is marked with an asterisk. Arrows indicate dorsal (D), ventral (V), anterior (A) and posterior (P).(A) Frizzled 5/8 across the apical plate, 16 hours post-fertilization (hpf) apical view and a(A’) 20 hpf ventral view (inset shows a surface ventral view).(B) Wnt4 along the blastopore, 16 hpf lateral view.(B’) Confocal image of ventral view of Wnt4 expression in which antibody staining to acetylated tubulin is visible with a green secondary antibody, reflection signal of in situ hybridization is red and nuclei are visible, stained with DAPI, in blue.(C) Apical view of sfrp1/5 expression at 24 hpf and at(C’) 48 hpf.(A”,B”and C”) Schematic representations of the expression patterns in(A) and(B) and(C) and(A’) and(B’) and(C’).
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