The plant tissues applied here are the whole abovegroundbiomass, for spearmint, and the upper part of shoots, for sage. Thesewere purchased from a commercial supplier. Plant tissues were cutinto small pieces, air-dried in the dark till moisture content wasapproximately 5–7%, and stored in a cool (12◦C), dark and dry placeuntil use. Storage duration varied from a few days to a few months,given that the whole experiment had to be repeated in time.To estimate the essential oil content of the two aromatic plants, aquantity of the plant material purchased was water-distilled whendry (100 g each time) for 3 h in a Clevenger apparatus. The essentialoils thus extracted from each species were analyzed by gas chro-matography as described by Vokou et al. (1993) and by GC–MS asdescribed by Karamanoli et al. (2008). The major constituents ofthese essential oils were identified on the basis of the retentiontimes and according to the chemical profiles found by Karousouet al. (1998) and Kokkini and Vokou (1989). The essential oil yield ofspearmint was 1.6 ml 100 g−1d.w., that of sage 1.5 ml 100 g−1d.w.Carvone (>50%) and 1,8-cineol (>40%) were the major constituentsof the essential oils of spearmint and sage, respectively.The above aromatic plants were applied as soil amendmentsin tomato seedbeds. Seeds (84% germination) of Carla F1 hybridtomato (Lycopersicon esculentum L.) were used in the experiments
The plant tissues applied here are the whole abovegroundbiomass, for spearmint, and the upper part of shoots, for sage. Thesewere purchased from a commercial supplier. Plant tissues were cutinto small pieces, air-dried in the dark till moisture content wasapproximately 5–7%, and stored in a cool (12◦C), dark and dry placeuntil use. Storage duration varied from a few days to a few months,given that the whole experiment had to be repeated in time.To estimate the essential oil content of the two aromatic plants, aquantity of the plant material purchased was water-distilled whendry (100 g each time) for 3 h in a Clevenger apparatus. The essentialoils thus extracted from each species were analyzed by gas chro-matography as described by Vokou et al. (1993) and by GC–MS asdescribed by Karamanoli et al. (2008). The major constituents ofthese essential oils were identified on the basis of the retentiontimes and according to the chemical profiles found by Karousouet al. (1998) and Kokkini and Vokou (1989). The essential oil yield ofspearmint was 1.6 ml 100 g−1d.w., that of sage 1.5 ml 100 g−1d.w.Carvone (>50%) and 1,8-cineol (>40%) were the major constituentsof the essential oils of spearmint and sage, respectively.The above aromatic plants were applied as soil amendmentsin tomato seedbeds. Seeds (84% germination) of Carla F1 hybridtomato (Lycopersicon esculentum L.) were used in the experiments
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