Collagen represents 30% of total protein in animals, and glycine and proline account for about 50% of the amino acids in collagen. Other amino acids contributing significantly to collagen in beef (in varying amounts) include alanine, arginine, glutamic acid, aspartic acid, leucine, lysine and serine (Bolboaca & Jantschi, 2007). It could be that more high nitrogen-containing amino acids are found in the hind shin, increasing total protein as determined by nitrogen content to inaccurate amounts, or the high concentrations of collagen and elastin could have restricted homogenous sampling and complicated hydrolysis during amino acid determination. Furthermore, haemoglobin and myoglobin, which are found in blood, contain noteworthy amounts of nitrogen. These free nitrogen molecules could also increase the total nitrogen content in the sample, inaccurately overestimating total protein. Sosulski and Imafidon (1990) found that nitrogen-to-protein conversion
factors for egg, meat, fish and cereal products varied between 5.61 and 5.93, and suggested that these lower values compared to the Jones factor, could be due to incomplete recovery of amino acids, amino acid losses during hydrolysis or the presence of nucleic acid nitrogen and non-amino-acid nitrogen molecules. Similarly, the current study calculated the nitrogen-to-protein conversion factor for South African beef to be on average 5.74 (Table 4), based on total amino acid content (g) divided by total nitrogen (g).
Collagen represents 30% of total protein in animals, and glycine and proline account for about 50% of the amino acids in collagen. Other amino acids contributing significantly to collagen in beef (in varying amounts) include alanine, arginine, glutamic acid, aspartic acid, leucine, lysine and serine (Bolboaca & Jantschi, 2007). It could be that more high nitrogen-containing amino acids are found in the hind shin, increasing total protein as determined by nitrogen content to inaccurate amounts, or the high concentrations of collagen and elastin could have restricted homogenous sampling and complicated hydrolysis during amino acid determination. Furthermore, haemoglobin and myoglobin, which are found in blood, contain noteworthy amounts of nitrogen. These free nitrogen molecules could also increase the total nitrogen content in the sample, inaccurately overestimating total protein. Sosulski and Imafidon (1990) found that nitrogen-to-protein conversionfactors for egg, meat, fish and cereal products varied between 5.61 and 5.93, and suggested that these lower values compared to the Jones factor, could be due to incomplete recovery of amino acids, amino acid losses during hydrolysis or the presence of nucleic acid nitrogen and non-amino-acid nitrogen molecules. Similarly, the current study calculated the nitrogen-to-protein conversion factor for South African beef to be on average 5.74 (Table 4), based on total amino acid content (g) divided by total nitrogen (g).
การแปล กรุณารอสักครู่..
Collagen represents 30% of total protein in animals, and glycine and proline account for about 50% of the amino acids in collagen. Other amino acids contributing significantly to collagen in beef (in varying amounts) include alanine, arginine, glutamic acid, aspartic acid, leucine, lysine and serine (Bolboaca & Jantschi, 2007). It could be that more high nitrogen-containing amino acids are found in the hind shin, increasing total protein as determined by nitrogen content to inaccurate amounts, or the high concentrations of collagen and elastin could have restricted homogenous sampling and complicated hydrolysis during amino acid determination. Furthermore, haemoglobin and myoglobin, which are found in blood, contain noteworthy amounts of nitrogen. These free nitrogen molecules could also increase the total nitrogen content in the sample, inaccurately overestimating total protein. Sosulski and Imafidon (1990) found that nitrogen-to-protein conversion
factors for egg, meat, fish and cereal products varied between 5.61 and 5.93, and suggested that these lower values compared to the Jones factor, could be due to incomplete recovery of amino acids, amino acid losses during hydrolysis or the presence of nucleic acid nitrogen and non-amino-acid nitrogen molecules. Similarly, the current study calculated the nitrogen-to-protein conversion factor for South African beef to be on average 5.74 (Table 4), based on total amino acid content (g) divided by total nitrogen (g).
การแปล กรุณารอสักครู่..