The molecular weight of purified protease was assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) using 5 % stacking and 12 % resolving gel and the protein was visualized by staining with coomassie brilliant blue R 250
The molecular weight of purified protease was assessed bysodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 5 % stacking and 12 % resolving gel andthe protein was visualized by staining with coomassie brilliant blue R 250