No. P6793; PM); Mitra et al. (1976; M) and Knudson ‘C’
(1946; KC) supplemented with BAP (0.5–2.0 mgl−1) and 2,4-
D (0.5–2.0 mgl−1), either individually or in combinations, two
additives such as peptone/activated charcoal were used for
seed germination. Hundred milliliters of medium were dispensed into 250 ml Erlenmeyer flasks and autoclaved at
121 °C for 20 min at 15 psi. Culture vessels with inoculated
seeds were maintained in a culture room where a cycle of 14/
10 h light–dark at 60 μmolm−2 s−1 provided by cool white
fluorescent lamps (Philips Truelight 36w/86 6500 °K B7,
Philips India), and 60 % relative humidity at 25±2 °C.