Similarly, there was a significant difference between the number of healthy bacteria
in the 0.7 μm-filtered OJ and both 0.22 μm-filtered and 1.2 μm-filtered OJ after 24 hour incubation at 22.5 °C. This
indicated that OJ cloud between 0.7 μmand 0.22 μmin sizemight have an adverse effect on the viability of E. coli
K-12. Furthermore, FCM allowed the rapid analysis of bacterial physiology without the requirement for growth
on agar plates, and revealed the extent of the viable but non-culturable (VBNC) population.