Solvent-solvent partitioning was done using the protocol
designed by Kupchan (1973) and modified version of Van
Wagenen (1993). Given that the sample containing ethanol
extract of barks was the best extract amongst all the crude
extracts, it was chosen for fractionation into polar, semi
polar and non polar fractions. Firstly, ethanol extract was
soaked in hexane solvent and was separated into hexane
fraction and hexane insoluble fraction. Secondly, hexane
insoluble fraction was partitioned with water and ethyl
acetate in 1:1 ratio. To carry out the bioactivity tests, the
resulting fractions were filtered through a fresh cotton plug
followed by a Whatman No.1 filter paper and evaporated
using a rotary evaporator at low temperature (40-45°C) and
pressure.