2.1 Preparation of Water Extract of Fresh C. campestris
A total of 40g of fresh C. campestris was cut into small pieces 1-2 cm and then macerated with a blender. These
materials were then soaked in 8L of distilled water for 24h at 25 °C in a shaker, and placed on a shaker (Faravani
et al. 2008). The mixture was then filtered through four layers of cheesecloth. The supernatant was filtered again
using paper “Whatman no. 42” as a stock solution for water extract of fresh C. campestris (40g/8L= 5000ppm).