Cytokeratin-stained cells were compared to nonstained
cells, and a fibroblast culture of fetal bovine cartilage was
used as a negative control, and nonspecific bindingreactions of the secondary and tertiary antibodies were
excluded. Morphologic evaluation was performed by an
inverted light microscope, and the imaging software of
CellD* imaging was used to assess the ratio of cytokeratinpositive
and cytokeratin-negative cells.