All fractionation steps were performed at room temperature unless otherwise specified. At each step, the inhibitory activity of DPP IV was measured in 50 mMTriseHCl buffer (pH 9.0) using Gly- Pro-MCA as a substrate.
All fractionation steps were performed at room temperature unless otherwise specified. At each step, the inhibitory activity ofDPP IV was measured in 50 mMTriseHCl buffer (pH 9.0) using Gly- Pro-MCA as a substrate.