using a Tensor 27
FTIR spectrometer (Bruker Optics) coupled to a HTS-XT
high-throughput device (Bruker Optics). Prior to identification,
spectra were pre-processed according to Oberreuter
et al. (2002). Identification was performed using the OPUS
5.5 software package (Bruker), in combination with libraries
of reference spectra. Isolates that displayed FTIR spectra that
did not match any reference in the libraries were identified
by gene sequence analysis before the corresponding spectra
were integrated into the system. During the course of this
study, three novel strains, designated strains WS4599,
WS4601T and WS4602, were identified as being members
of the genus Micrococcus. Strains WS4601T and WS4602
were isolated from the examination room and cloakroom
of a single medical practice, respectively, whereas strain
WS4599 was isolated from a pharmaceutical clean room.