Yeast strains can be stored at -70°C in 15% glycerol and are viable for morethan 3 year. alternatively, they can be stored at 4°C on slants of rich medium for 6 month to 1 year. According to the information of CARS for our process the yeast medium is developed with the simple general condition. After adding with the solution a fermentation time is required for the ethanol production. In our study we have made three samples by varying the fermentation time as 3 days, 5 days and 7 days.