However, since nutritional needs change through the larval development, the precocious detection of skeletal deformities could aid to determine the influence nutrients on early larval development. In this sense, the establishment of the alcian blue-alizarin red double staining technique for developing European sea bass larvae becomes useful to describe skeletogenesis as well as to evaluate any factor that could induce skeletal deformities. Although the ontogeny of the cephalic
preserved in fixative solution