Determination of Antimicrobial Activity of LAB isolates against S.aureus
The antimicrobial activity of isolated LAB S.aureus was performed by well-diffusion method[5]. S.aureus was
incubated in BHI broth at 37° C at 24 hrs. 15ml of Muller Hinton Agar was prepared and 150μl of S.aureus culture
having 6 × 108 CFU/ml was inoculated into it. Once solidified the plates, wells of 6mm diameter were made and
40μl of each concentration of LAB isolates were filled into well [6]. Then the plates were incubated at 37°C for 24
hrs. And antimicrobial activity was determined by measuring the clear zone around the well [7].