After 60 days, the seedlings were transferred to 80 ml of 1/2 MS medium in 250 ml Erlenmeyer flasks (Murashige and Skoog, 1962), and subcultured for two months in the same condition
After 60 days, the seedlings were transferred to 80 ml of 1/2 MS medium in250 ml Erlenmeyer flasks (Murashige and Skoog, 1962), and subculturedfor two months in the same condition